ABSTRACT
In many countries, homeopathic preparation is believed to be an effective medicine for various ailments.However, there are limited scientific evidences in regard to its usage, safety, and efficacy. It is necessary toupdate this age-old scientific wisdom in different aspects, including pharmacologic and therapeutic potentials.In this study, we assessed the safety profile of homeopathic preparation of Gymnema sylvestre (HPGS). Itschemical constituents were deciphered using LCMS approaches. HPGS was subjected to an acute toxicitystudy (OECD-423 guidelines) using Sprague Dawley rats. The administration of HPGS did not produce anytoxic symptoms or show mortality at the dose level of 300 mg/kg body weight. Phytochemical analysis revealedthat HPGS contained alkaloids, saponins, and flavonoids. These results demonstrated the non-toxic nature ofHPGS in vivo, suggesting a long-term usage in clinical practices when administered orally